Light sheet microscopy, also known as selective plane illumination microscopy (SPIM), is a powerful imaging technique used in biological research to capture high-resolution, three-dimensional images of biological samples. Light sheet microscopy works by illuminating a thin sheet of laser light perpendicular to the imaging plane, which reduces phototoxicity and photobleaching compared to traditional microscopy techniques. This allows for long-term imaging of live samples, making it ideal for studying dynamic biological processes. Light sheet microscopy has been used to study a wide range of biological phenomena, from embryonic development to neuronal activity in the brain.